Details-enriched characterization of mobile populations. Morphological information and facts from pictures can include for the richness of flow cytometry knowledge. For instance, the determine demonstrates an otherwise typical apoptosis assay making use of Annexin V and PI, incorporating mobile imaging to characterize cells in Every population to expose morphologically distinct functions. These insights could not are actually gained from multiplex staining by itself.
As samples are obtained about the Attune CytPix Flow Cytometer, the significant-pace brightfield digital camera captures and suppliers pictures of detected gatherings in a charge around six,000 illustrations or photos per 2nd, depending on the flow amount and image measurement. For increased adaptability, Attune Cytometric Software lets you alter the picture capture frequency as needed and select a selected gate for graphic capture, if essential.
Mix acoustic concentrating technology with large-parameter analysis for flexible spectral unmixing and standard workflows.
The award-successful Invitrogen Attune CytPix Flow Cytometer brings together acoustic focusing having a large-velocity brightfield camera for simultaneous significant throughput flow cytometry and significant resolution brightfield imaging.
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In this article, a qualified user has gated singlets confidently. Right after assessing the handbook singlet gate, the CytPix graphic-derived parameter ParticleCount reveals this gate is made up of much more than 4% aggregates.
Pair your Attune flow cytometer with the autosampler for enhanced workflow effectiveness, and add robotic integration for max throughput.
Regular graphic good quality even at higher throughput Acoustic concentrating in addition to a significant-velocity camera Merge to image these T cells consistently at lower or large flow charges. Very easily change focus and camera settings to meet experimental requirements.
Early signs of this contamination experienced initially been dismissed as particles. Backgating to find new populations. Backgating imaged cells within the Attune CytPix Flow Cytometer also helps you to use morphological options to discover appealing subpopulations that might not be apparent from flow cytometry info by itself.
End users can perspective event pictures in true-time to confirm sample top quality, improve protocols, and report the morphology of each cell populace identified for Assessment. Imaging capture also Positive aspects from acoustic focusing, which really helps to place the cells to ensure that a sharp, centered image is obtained.
AI-driven automatic graphic Evaluation While using the Attune Cytometric Software package interprets party characteristics into distinctive morphology parameters that can be coupled with common fluorescence and scatter parameters to reveal extremely reproducible inhabitants adjustments and unique subsets.
We Beforehand shown the strength of imaging Vehicle-T/Ramos cell interactions. Permit’s check out just the inhabitants of biggest curiosity, the double beneficial functions, To find out more. We could now use extended morphological parameters (here, circularity and skewness of depth) to more take a look at the capabilities of such populations and refine gating on these gatherings, expanding info robustness.
Leverage the power of State-of-the-art engineering and innovation to accelerate your discoveries. Have faith in while in the Attune Flow Cytometer spouse and children to deliver the reliability, pace, and accuracy that established your work aside.
Adaptable payment approach from Agilent Monetary Options help you have the important instrumentation you may need for fast challenge, or to increase your lab.
At the center of each Attune Flow Cytometer lies our innovative acoustic concentrating know-how, which utilizes seem waves to meticulously align cells within the flow cell. This modern technique surpasses conventional hydrodynamic techniques, leading to more quickly processing speeds, exceptional details good quality, and elevated sample throughput.
As the cell line had Earlier been analyzed to the Attune CytPix Flow Cytometer, the scientists went again to the photographs and had been capable of document the microbial an infection at least 5 days previously.
The Attune CytPix Flow Cytometer makes it possible for end users to visualize https://anotepad.com/notes/sm7x77fa samples for the duration of acquisition, validate the precision of gating techniques, and assist effects with dependable documentation for publication reasons.
What gave the impression to be a homogeneous inhabitants is actually much more various—an Perception that ought to be deemed when interpreting final results.